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biotin conjugated maa ii b 1265  (Vector Laboratories)


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    Vector Laboratories biotin conjugated maa ii b 1265
    Biotin Conjugated Maa Ii B 1265, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/maackia+amurensis+lectin+ii+mal+ii+biotin/Biotinylated+Maackia+Amurensis+Lectin+II+(MAL+II)/pmc13094297-288-24-18
    Average 96 stars, based on 426 article reviews
    biotin conjugated maa ii b 1265 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Suspension:

    Article Title: Massively parallel interrogation of human functional variants modulating cancer immunosurveillance
    Article Snippet: .. The Maackia Amurensis Lectin II (MAL-II)-biotin (Vector Laboratories, #B-1265) was added to the suspension at a final concentration of 5 μg/mL, followed by incubation at room temperature for 30 min. Next, the cells were washed with DPBS three times and stained with 1 μg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min. After washing with DPBS three times, flow cytometry analysis was performed to detect the AF647 (APC) signal with the BD LSRFortessa SORP (BD Biosciences). .. The supernatant was collected with 30 μL of the cell lysates as the input, and the rest was treated with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, #A2220) or HA beads (Sigma-Aldrich, #E6779) at 4°C overnight.

    Concentration Assay:

    Article Title: Massively parallel interrogation of human functional variants modulating cancer immunosurveillance
    Article Snippet: .. The Maackia Amurensis Lectin II (MAL-II)-biotin (Vector Laboratories, #B-1265) was added to the suspension at a final concentration of 5 μg/mL, followed by incubation at room temperature for 30 min. Next, the cells were washed with DPBS three times and stained with 1 μg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min. After washing with DPBS three times, flow cytometry analysis was performed to detect the AF647 (APC) signal with the BD LSRFortessa SORP (BD Biosciences). .. The supernatant was collected with 30 μL of the cell lysates as the input, and the rest was treated with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, #A2220) or HA beads (Sigma-Aldrich, #E6779) at 4°C overnight.

    Incubation:

    Article Title: Massively parallel interrogation of human functional variants modulating cancer immunosurveillance
    Article Snippet: .. The Maackia Amurensis Lectin II (MAL-II)-biotin (Vector Laboratories, #B-1265) was added to the suspension at a final concentration of 5 μg/mL, followed by incubation at room temperature for 30 min. Next, the cells were washed with DPBS three times and stained with 1 μg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min. After washing with DPBS three times, flow cytometry analysis was performed to detect the AF647 (APC) signal with the BD LSRFortessa SORP (BD Biosciences). .. The supernatant was collected with 30 μL of the cell lysates as the input, and the rest was treated with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, #A2220) or HA beads (Sigma-Aldrich, #E6779) at 4°C overnight.

    Staining:

    Article Title: Massively parallel interrogation of human functional variants modulating cancer immunosurveillance
    Article Snippet: .. The Maackia Amurensis Lectin II (MAL-II)-biotin (Vector Laboratories, #B-1265) was added to the suspension at a final concentration of 5 μg/mL, followed by incubation at room temperature for 30 min. Next, the cells were washed with DPBS three times and stained with 1 μg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min. After washing with DPBS three times, flow cytometry analysis was performed to detect the AF647 (APC) signal with the BD LSRFortessa SORP (BD Biosciences). .. The supernatant was collected with 30 μL of the cell lysates as the input, and the rest was treated with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, #A2220) or HA beads (Sigma-Aldrich, #E6779) at 4°C overnight.

    Flow Cytometry:

    Article Title: Massively parallel interrogation of human functional variants modulating cancer immunosurveillance
    Article Snippet: .. The Maackia Amurensis Lectin II (MAL-II)-biotin (Vector Laboratories, #B-1265) was added to the suspension at a final concentration of 5 μg/mL, followed by incubation at room temperature for 30 min. Next, the cells were washed with DPBS three times and stained with 1 μg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min. After washing with DPBS three times, flow cytometry analysis was performed to detect the AF647 (APC) signal with the BD LSRFortessa SORP (BD Biosciences). .. The supernatant was collected with 30 μL of the cell lysates as the input, and the rest was treated with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, #A2220) or HA beads (Sigma-Aldrich, #E6779) at 4°C overnight.

    other:

    Article Title: Massively parallel interrogation of human functional variants modulating cancer immunosurveillance.
    Article Snippet: Flow cytometry analysis was performed with the BD LSRFortessa SORP (BD Biosciences).

    Article Title: A Comparative Mechanistic Study of Live-Cell Glycocalyx Engineering: Improving Adoptive Cell Therapies Against B Lymphoma
    Article Snippet: Aleuria aurantia lectin (AAL)-biotin, Sambucus nigra lectin (SNA)-biotin, and Maackia amurensis lectin II (MAL-II)-biotin were purchased from VectorLabs (USA).

    Article Title: Massively parallel interrogation of human functional variants modulating cancer immunosurveillance
    Article Snippet: For each group, all extracted genomes were used as the PCR templates and the sgRNA-coding sequences with iBAR were amplified using KAPA HiFi HotStart ReadyMix PCR kit (Roche, #KK2631).



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    α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections <t>using</t> <t>MAL</t> <t>II</t> lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .
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    Image Search Results


    α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections using MAL II lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .

    Journal: Frontiers in Pharmacology

    Article Title: Mycobacterium tuberculosis infection drives osteoclast overactivation via α2,3-Sialylation to promote pathological bone destruction

    doi: 10.3389/fphar.2026.1738896

    Figure Lengend Snippet: α2,3-sialylation is required for BCG-induced osteoclast differentiation and activity (A) Comparison of differentially expressed genes (DEGs) between BCG-infected osteoclasts (RB) and uninfected controls (R). (n = 3 biological replicates per group, differential expression was defined as |FoldChange| > 2 and padj <0.05). (B) Volcano plot of DEGs highlighting genes related to sialic acid biosynthesis. (C) KEGG pathway enrichment analysis of upregulated DEGs in RB cells. (D,E) Immunofluorescence staining of α2,3-SA in mouse calvarial sections using MAL II lectin, with quantification of α2,3-SA fluorescence intensity (n = 5). Intensity density was normalized to the PBS group mean. (F) In vitro osteoclasts subjected to MAL II lectin staining and TRAP staining, with or without sialidase treatment to enzymatically remove α2,3-SA. (G) Quantification of α2,3-SA fluorescence intensity in cultured osteoclasts (n = 5). Intensity density was measured in cellular ROIs after background subtraction and normalized to the RANKL group mean. (H) Quantification of TRAP + multinucleated cells (≥3 nuclei) per field (randomly selected fields, fixed magnification) in vitro (n = 5). (I) mRNA expression levels of osteoclast differentiation markers ( Fos, Mmp9, Nfatc1, and Ocstamp ) in osteoclasts (n = 3). Data are presented as mean ± SD. Statistical significance was determined by two-tailed unpaired Student’s t-test for two-group comparisons (E) and one-way ANOVA followed by Tukey’s post hoc test for three-group comparisons (G–I) .

    Article Snippet: Biotin MAL-II , Vector laboratories , Cat# B-1265-1.

    Techniques: Activity Assay, Comparison, Infection, Quantitative Proteomics, Immunofluorescence, Staining, Fluorescence, In Vitro, Cell Culture, Expressing, Two Tailed Test